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cells  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec cells
    Cells, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 230 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/foxp3+staining+buffer/FoxP3+Staining+Buffer+Set/pm42401537-343-3-9
    Average 97 stars, based on 230 article reviews
    cells - by Bioz Stars, 2026-09
    97/100 stars

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    Related Articles

    Staining:

    Article Title: E-cadherin expression promotes tumor growth via KLRG1-dependent pathways
    Article Snippet: Included in all antibody staining cocktails was an anti-CD16/CD32 (clone 2.4G2; BioXCell; Cat#BE0307, RRID: AB_2736987) blocking antibody and brilliant violet staining buffer (BD Biosciences; Cat#563794). .. Nuclear staining was performed using the FoxP3 Staining Buffer Set (Miltenyi; Cat130-093-142). .. Antibodies used for flow cytometry: from Invitrogen, FoxP3-PE-Cy5.5 (Cat# 35-5773-80; RRID: AB_468806); from BioLegend, PD-1-PE (Cat# 135206; RRID: AB_1877231), E-Cadherin-BV421 (Cat# 147319; RRID: AB_2750483), CD45-BV570 (Cat# 103136; RRID: AB_2562612), and TIM-3-BV785 (Cat# 119725; RRID: AB_2716066); from BD, CD3e-BUV395 (Cat# 563565; RRID: AB_2738278), TCRb-BUV496 (Cat# 749915; RRID: AB_2874154), NK1.1-BUV615 (Cat# 751111; RRID: AB_2875140), KLRG1-BV421 (Cat# 562897; RRID: AB_2737875), CD8-BUV805 (Cat# 612898; RRID: AB_2870186), Ly-108-BV650 (Cat# 740628; RRID: AB_2740323), KLRG1-BV711 (Cat# 564014; RRID: AB_2738542), CD44-FITC (Cat# 553133; RRID: AB_2076224), and CD4-BUV563 (Cat# 612923; RRID: AB_2870208).

    Article Title: IL-10 secretion by CD8 T cells orchestrates early NK cell recruitment and antitumor immunity after anti-VEGFR-2/PD-1 therapy.
    Article Snippet: Then, cell surface markers labeling was performed 15 min in the dark at 4°C in Brilliant Stain Buffer (566349, BD Biosciences) using antibodies diluted at 1/50e. .. Intracellular staining was performed after fixation and permeabilization of the cells using the Foxp3 Staining Buffer Set (130–093- 142, Miltenyi Biotec) for 30 min. ..

    Article Title: Cytokine-bearing Bacterial Outer Membrane Vesicles with Empowered Efficacy in Intratumoral Immunotherapy
    Article Snippet: .. After two washes with PBS, cells were fixed using Cytofix reagent (BD Bioscience, San Jose, CA, USA) for protocol B or with the Fixation/Permeabilization Solution of FoxP3 Staining Buffer Set (Miltenyi Biotech, Bergisch Gladbach, Germany) for protocol A. .. In this later case, after fixation cells were also incubated with an anti-FoxP3-PE (Miltenyi Biotech, Bergisch Gladbach, Germany) antibody diluted in permeabilization buffer and stained for 20 minutes at RT.

    Article Title: Multi-compartment immune cell profiling highlights the prognostic relevance of CD127+ CD8+ T cells for patients with high-grade serous ovarian cancer
    Article Snippet: .. In terms of FoxP3 staining, cells were fixed and permeabilized using FoxP3 staining buffer set according to manufacturer’s instructions (Miltenyi Biotec) followed by an incubation step (30 min, 4 °C, dark) with fluorophore-labeled anti-human FoxP3 antibody or isotype control ( ). .. On the other hand, cells were incubated with 4% paraformaldehyde solution (PFA, 10 min, on ice, dark, Sigma-Aldrich) and 0.1% saponin solution (3 min, 4 °C, dark, Carl Roth GmbH + Co. KG, Karlsruhe, Germany) for the intracytoplasmic staining of myeloperoxidase (MPO) and arginase 1 in the neutrophil staining panel (antibody dilution in saponin buffer).

    Article Title: Generation of human induced pluripotent stem cell line from an obesity patient with POMC deficiency due to POMC:W84X mutation.
    Article Snippet: .. Cells were fixed and permeabilised using FoxP3 Staining Buffer Set (Miltenyi). ..

    Article Title: Generation of four human induced pluripotent stem cell lines from functionally distinct T cell lineages with defined or undefined T cell receptor specificity.
    Article Snippet: For FC staining, cells were harvested at passage numbers 21, 14, 19, 21 for BIHi289–C, BIHi289–E, BIHi290–A, BIHi290–D, respectively, using TrypLE Select (Thermo) and 2 × 105 live cells were incubated with TRA-1–60 and SSEA-4 antibodies for 10 min at 4 ◦C in 100 μL staining buffer (DPBS, 2 mM EDTA, 0.5 % BSA). .. Cells were fixed and permeabilised using FoxP3 Staining Buffer Set (Miltenyi Biotec). ..

    Article Title: Generation of two human iPSC lines from fibroblasts of BPAN patients carrying pathogenic variants in the WDR45 gene
    Article Snippet: .. The cells were fixed and permeabilized using the FoxP3 Staining Buffer Set (Miltenyi, #130-093-142). ..

    Article Title: B cell-intrinsic IL-2 signaling regulates inflammation by promoting IL-10 expression in CD25 + age-associated B cells.
    Article Snippet: Intracellular transcription factor staining Cells were first labeled with a viability marker (Live dead, Thermo Fisher Scientific, L34994) and then incubated with FcR Blocking Reagent for 10 minutes before membrane staining described above. .. Cells were fixed and permeabilized with FoxP3 Staining buffer ll OPEN ACCESS Article e8 Immunity 59, 1–18.e1–e9, February 10, 2026 Set (Miltenyi Biotec, 130-093-142) or Transcription factor staining buffer set (Miltenyi Biotec, 130-122-981) and stained with mixture of fluorescent antibodies for 30 minutes at 4◦C in the dark. ..

    Incubation:

    Article Title: Multi-compartment immune cell profiling highlights the prognostic relevance of CD127+ CD8+ T cells for patients with high-grade serous ovarian cancer
    Article Snippet: .. In terms of FoxP3 staining, cells were fixed and permeabilized using FoxP3 staining buffer set according to manufacturer’s instructions (Miltenyi Biotec) followed by an incubation step (30 min, 4 °C, dark) with fluorophore-labeled anti-human FoxP3 antibody or isotype control ( ). .. On the other hand, cells were incubated with 4% paraformaldehyde solution (PFA, 10 min, on ice, dark, Sigma-Aldrich) and 0.1% saponin solution (3 min, 4 °C, dark, Carl Roth GmbH + Co. KG, Karlsruhe, Germany) for the intracytoplasmic staining of myeloperoxidase (MPO) and arginase 1 in the neutrophil staining panel (antibody dilution in saponin buffer).

    Control:

    Article Title: Multi-compartment immune cell profiling highlights the prognostic relevance of CD127+ CD8+ T cells for patients with high-grade serous ovarian cancer
    Article Snippet: .. In terms of FoxP3 staining, cells were fixed and permeabilized using FoxP3 staining buffer set according to manufacturer’s instructions (Miltenyi Biotec) followed by an incubation step (30 min, 4 °C, dark) with fluorophore-labeled anti-human FoxP3 antibody or isotype control ( ). .. On the other hand, cells were incubated with 4% paraformaldehyde solution (PFA, 10 min, on ice, dark, Sigma-Aldrich) and 0.1% saponin solution (3 min, 4 °C, dark, Carl Roth GmbH + Co. KG, Karlsruhe, Germany) for the intracytoplasmic staining of myeloperoxidase (MPO) and arginase 1 in the neutrophil staining panel (antibody dilution in saponin buffer).



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